They are accused of paying out more than $2m (£1.5m) in compensation for land which already belonged to the government.
The individuals who allegedly received the illegal payments have also been charged.
All of the accused have pleaded not guilty.
The land was acquired for a $3bn Chinese-funded railway that links the capital Nairobi with the port city of Mombasa.
President Uhuru Kenyatta has said he is willing to lose friends in order to end impunity in Kenya.
